Journal: Pharmaceuticals

Article Title: Hydrophilic and Lipophilic Carbon Dots Impart Thermosensitivity to Doxorubicin Loaded Phospholipid Liposomes

doi: 10.3390/ph19050668

Figure Lengend Snippet: Comparative cytotoxicity of either empty or DOX-loaded PEGylated DPPC liposomes (Lip), or CD (Lip-CD) and CD-C16 (Lip-CD16) containing liposomes against PC3 cells following an 1 h incubation at ( A ) 37 or ( B ) 40°C. DOX concentration was 5 or 10 μM, while the concentration of empty liposomes corresponded to the liposomal concentration in experiments at the highest DOX concentration (10 μΜ). The cytotoxicity was assessed after a 24 h post-incubation period employing the standard MTT assay. Cell survival data are the mean of at least six independent values while error bars represent one standard deviation. The statistical significance was derived by Student’s t -test following the assignment: * p < 0.05, ** p < 0.01, *** p < 0.001; ns denotes no statistical significance, p > 0.05.

Article Snippet: The human prostate cancer PC3 cell line as well as the non-cancerous human embryonic kidney HEK293 of the cell bank from the Institute of Nanoscience and Nanotechnology, NCSR Demokritos, were purchased from the American Type Culture Collection (ATCC CRL-1435TM and CRL-1573TM, respectively; Manassas, VA, USA).

Techniques: Liposomes, Incubation, Concentration Assay, MTT Assay, Standard Deviation, Derivative Assay

Journal: Frontiers in Immunology

Article Title: PhIP-driven prostate cancer involves key molecular regulators and immune microenvironment modulation

doi: 10.3389/fimmu.2026.1782240

Figure Lengend Snippet: PhIP exposure induces cytotoxicity in RWPE-1 cells and downregulates SLC14A1 expression in PC-3 cells. (A) Immunohistochemical staining of SLC14A1 in prostate cancer tissues and normal prostate tissues based on data from the HPA database. (B) Cell viability of RWPE-1 cells after treatment with increasing concentrations of PhIP for 48 h, as determined by CCK-8 assay. (C) Protein expression levels of SLC14A1 in PC-3 cells following PhIP treatment, as assessed by Western blot. (D) Relative mRNA expression levels of SLC14A1 in PC-3 cells after PhIP treatment, as determined by qRT-PCR (**p < 0.01, ***p < 0.001).

Article Snippet: The human prostatic epithelial cell line RWPE-1 and the human prostate cancer cell line PC-3 were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Immunohistochemical staining, Staining, CCK-8 Assay, Western Blot, Quantitative RT-PCR

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of different concentration of capsaicin on cell viability. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentrations of capsaicin for 24 and 48 h. The viability of cells was determined by MTT cell viability assay and expressed as percentage of the control. Data are shown as mean ± SD; * p < 0.05 compared with the control group.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Concentration Assay, Viability Assay, Control

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of different concentrations of ALA on cell viability. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentrations of ALA for 24 and 48 h. The viability of cells was determined by MTT cell viability assay and expressed as percentage of the control. Data are shown as mean ± SD; * p < 0.05 compared with the control group.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Viability Assay, Control

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of different concentrations of capsaicin and ALA combination on cell viability. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentrations of capsaicin and ALA for 24 and 48h. The viability of cells was determined by MTT cell viability assay and expressed as percentage of the control. Data are shown as mean ± SD; * < 0.05 compared with the control group; # < 0.05 compared with the lowest concentration of ALA (250 µM)-treated cells.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Viability Assay, Control, Concentration Assay

Journal: Current Issues in Molecular Biology

Article Title: In Silico and In Vitro Investigation of Apoptosis-Mediated Antiproliferative Activity of Capsaicin and Alpha-Lipoic Acid Against Prostate Cancer Cells

doi: 10.3390/cimb48040376

Figure Lengend Snippet: Effect of capsaicin, ALA and their combination on apoptosis-related protein expression in prostate cancer cells. Two different prostate cancer cell lines (PC-3 and DU-145) were treated with different concentration of capsaicin, ALA and their combination for 24 h. Survivin and bax protein expressions were determined using the Western blotting method ( B ). β-actin is used as a loading control. Quantitative analysis of survivin ( A ) and bax ( C ) protein. * p < 0.05 compared with the control group.

Article Snippet: Prostate cancer cell lines PC-3 (CRL-1435) and DU-145 (HTB-81) were purchased from American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Concentration Assay, Western Blot, Control